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lines changed Original file line number Diff line number Diff line change @@ -110,7 +110,7 @@ def run_bowtie(args):
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write to files to save memory
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'''
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metagenome , fastqfile = args
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- b_opt = "-k 1 -p 1 --quiet --no-hd"
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+ b_opt = "-k 1 -p 1 --quiet --no-hd --no-unal "
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command = shlex .split (f"bowtie2 { b_opt } -x { metagenome } { fastqfile } " )
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bowtie_align = subprocess .run (command , check = True ,
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capture_output = True , text = True ).stdout
@@ -148,6 +148,7 @@ def run_bowtie(args):
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repeats_by_reads [read ].append (repname )
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for repname in repeats_by_reads :
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repeats_by_reads [repname ] = list (set (repeats_by_reads [repname ]))
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+ # this repeats_by_reads dictionary is far too big
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# 3 dictionnaries and 1 pointer variable to be populated
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fractionalcounts = defaultdict (float )
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