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nextflow.config
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manifest {
author = 'Dan Fornika <[email protected]>, Sherrie Wang <[email protected]>'
name = 'BCCDC-PHL/kma-cgmlst'
version = '0.2.1'
description = 'BCCDC-PHL kma cgMLST'
mainScript = 'main.nf'
nextflowVersion = '>=20.01.0'
}
params {
profile = false
illumina_suffixes = ['*_R{1,2}_001', '*_R{1,2}', '*_{1,2}' ]
nanopore_suffixes = ['*_RL']
fastq_exts = ['.fastq.gz', '.fq.gz', '.fastq', '.fq']
fastq_illumina_search_path = makeFastqSearchPath( params.fastq_input, illumina_suffixes, fastq_exts )
fastq_nanopore_search_path = makeFastqSearchPath( params.fastq_input_long, nanopore_suffixes, fastq_exts )
fastq_input = 'NO_FILE'
fastq_input_long = 'NO_FILE'
samplesheet_input = 'NO_FILE'
scheme = 'NO_FILE'
pipeline_short_name = parsePipelineName(manifest.toMap().get('name'))
pipeline_minor_version = parseMinorVersion(manifest.toMap().get('version'))
filtlong_min_length = 1000
filtlong_keep_percent = 90
min_identity = 100.0
min_coverage = 100.0
collect_outputs = false
collected_outputs_prefix = 'collected'
cache = ''
}
def makeFastqSearchPath ( base_path, fastq_suffixes, fastq_exts ) {
def fastq_search_path = []
for (suffix in fastq_suffixes){
for (ext in fastq_exts) {
fastq_search_path.add(base_path.toString() + '/' + suffix.toString() + ext.toString())
}
}
return fastq_search_path
}
def parseMinorVersion(version) {
minor_version = version.split('\\.')[0..1].join('.')
return minor_version
}
def parsePipelineName(name) {
short_name = name.split('/')[1]
return short_name
}
profiles {
conda {
conda.enabled = true
process.conda = "$baseDir/environments/environment.yml"
if (params.cache){
conda.cacheDir = params.cache
}
}
}
process {
withName: fastp {
cpus = 4
}
withName: kma_align {
cpus = 8
}
withName: filtlong {
conda = "$baseDir/environments/long_read_qc.yml"
}
}