What do we mean by quantitative bioimaging?
Is that question addressed at that page? I do not think so!
Nowhere in the document or in the publication is discussed what quantitative imaging offers in the sense of what can be quantified receptively measured with microscopes.
The very first thing to consider is what do I want to quantify:
- number of objects (cells, compartments, certain proteins or RNAs, ...)
- distances or areas (in 2D or 3D)
- volumes
- times (for replication, stress response, chemical reactions, ...)
- intensities (for counting, distance measurements as with FRET, potential measures, ...)
- ...
For all these options it is crucial to consider the necessary resolution (in space, time and/or color) for the experiment.
That is the base to choose the microscope that fits the purpose of the quantitative microscope experiment, which is the first step of planning to my experience.
All the points addressed on that page are as important as they are, not the first thing to consider planning your quantitative experiment.
What do we mean by quantitative bioimaging?
Is that question addressed at that page? I do not think so!
Nowhere in the document or in the publication is discussed what quantitative imaging offers in the sense of what can be quantified receptively measured with microscopes.
The very first thing to consider is what do I want to quantify:
For all these options it is crucial to consider the necessary resolution (in space, time and/or color) for the experiment.
That is the base to choose the microscope that fits the purpose of the quantitative microscope experiment, which is the first step of planning to my experience.
All the points addressed on that page are as important as they are, not the first thing to consider planning your quantitative experiment.