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Question: dump sequences of hetmers #185

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jts opened this issue Feb 3, 2025 · 2 comments
Open

Question: dump sequences of hetmers #185

jts opened this issue Feb 3, 2025 · 2 comments
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enhancement New feature or request

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@jts
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jts commented Feb 3, 2025

Hi,

Thanks for developing smudgeplot, I've found it to be very useful. I would like to do a deeper analysis of the results, which requires the sequences of each A/B hetmer (or a representative subsample) that smudgeplot finds. Is there an option or switch that will dump this output? Apologies if it has already been asked, I searched the issues but could not find anything.

Jared

@KamilSJaron
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Hi Jared,

thanks for kind words, really appreciated.

This is a feature we are working on with @thegenemyers.

If you have a smaller dataset you would like to try it with and not wait for the newer version, we had this implemented in some older versions (v0.2.5 was the last one). Although there was also a bit of a bug in the search - we did not search both canonical and non-canonical representations of each k-mers which means that the set of k-mer pairs is not really guaranteed to be complete complete, or always represent unique k-mer pairs (empirically we know that it is relatively sensible proxy though).

@jts
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jts commented Feb 4, 2025

Thanks for the fast reply. The genome I am working on is quite large, but I may try v0.2.5 anyway. Please do let me know when this is re-implemented and I will try the new version too.

Jared

@KamilSJaron KamilSJaron added the enhancement New feature or request label Feb 15, 2025
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