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Docs: changes, manual and version for 5.1.0.rc1
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CHANGES.txt

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a contig if it covers more than 95% of a cyclic chromosome/plasmid (prokaryotes only).
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4. Small fixes (rare crashes or slightly incorrect results in specific cases):
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- GC calculation;
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- duplication ratio calculation in case of Ns stretches in misassembled contigs;
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- HTML report heatmap colors for partial BUSCO genes;
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- use of provided BAM files;
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- problems with ?
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5. Cosmetic changes in warning/notice/error messages, pipeline steps order, etc.
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- GC calculation (zero division due to rate side effects);
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- duplication ratio (fractional overestimation due to Ns stretches in some contigs);
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- HTML report heatmap colors for partial BUSCO genes (red-blue color switch);
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- use of provided BAM files (--bam option not working properly);
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- postprocessing of minimap2 mediocre aligments (good pairs of alignments with a
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strecth of mismatches/indels/Ns in between were skipped due to low averageg IDY).
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5. Updates in embedded tools:
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- new version of SILVA database (138);
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- fixed links to BUSCO databases (v3/odb9);
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- new GeneMark license files.
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PLANNED TO BE INCLUDED IN 5.1.0:
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x. New metrics:
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- auNx, auNAx, auNGx, auNGAx (area under Nx, NGx, etc curves).
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6. Cosmetic changes in warning/notice/error messages, pipeline steps order, etc.
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x. Updates in embedded tools:
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- new version of SILVA database (now: 132);
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- new versions of BUSCO databases;
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- new GeneMark license files.
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5.0.2
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1. Fixed bug with missing genome features reference stats and plot in report.html

VERSION.txt

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5.1.0.dev0
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5.1.0rc1

manual.html

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for guidelines on how to complete the licensing process.
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<br>
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<br>
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Version 5.1.0 of QUAST was released under GPL v2 on April XX, 2020. Note that some of build-in third-party tools are not under GPL v2. See <a href="LICENSE.txt">LICENSE</a> for details.
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Version 5.1.0rc1 (release candidate) of QUAST was released under GPL v2 on April 29, 2020. Note that some of build-in third-party tools are not under GPL v2. See <a href="LICENSE.txt">LICENSE</a> for details.
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</p>
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<h2>Contents</h2>
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sudo apt-get install -y pkg-config libfreetype6-dev libpng-dev python-matplotlib
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</pre>
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<br>
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<!--
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To download the <a href="https://downloads.sourceforge.net/project/quast/quast-5.1.0.tar.gz">QUAST source code tarball</a> and extract it, type:
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<pre class="code">
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wget https://downloads.sourceforge.net/project/quast/quast-5.1.0.tar.gz
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tar -xzf quast-5.1.0.tar.gz
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cd quast-5.1.0
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</pre>
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-->
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To download the <a href="https://github.com/ablab/quast/releases/download/quast_5.1.0rc1/quast-5.1.0rc1.tar.gz">QUAST source code tarball</a> and extract it, type:
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<pre class="code">
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wget https://github.com/ablab/quast/releases/download/quast_5.1.0.rc1/quast-5.1.0rc1.tar.gz
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tar -xzf quast-5.1.0rc1.tar.gz
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cd quast-5.1.0rc1
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</pre>
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<br>
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<p>
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<ol class='my_ol' style='margin-top: 0;'>
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<li>For gene finding, GeneMark-ES is used with <code>--fungus</code> option.
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<li><a href='#rna_finding'>Barrnap</a> will use eukaryotic database to predict ribosomal RNA genes.
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<li>><a href='#busco'>BUSCO</a> will use fungal database to find conserved orthologs.
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<li><a href='#busco'>BUSCO</a> will use fungal database to find conserved orthologs.
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<li>By default, QUAST assumes that a genome is circular and correctly processes its linear representation.
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This options indicates that the genome is not circular.
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</ol>

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