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Update README.md
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@@ -7,17 +7,19 @@ Closely related host species share similar symbionts, but the effects of host ge
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## Repository structure
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data/ --> data necessary to run these scripts; these are a list of phyloseq objects for the wild dataset and lab dataset. Each phyloseq object corresponds to OTU table, taxonomic table and metadata for each amplicon, after identification and quality screening of ASVs and taxonomic annotation. Data description can be found here: https://doi.org/10.1186/s13071-023-05800-6
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### data/ --> data necessary to run these scripts; these are a list of phyloseq objects for the wild dataset and lab dataset. Each phyloseq object corresponds to OTU table, taxonomic table and metadata for each amplicon, after identification and quality screening of ASVs and taxonomic annotation. Data description can be found here: https://doi.org/10.1186/s13071-023-05800-6
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R/ --> scripts for data analysis
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### R/ --> scripts for data analysis
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tmp/ --> temporary files created from running the scripts
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### tmp/ --> temporary files created from running the scripts
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fig/ --> figures
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Briefly:
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## Brief description of each script:
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R/1_filtering.R --> Needs files data/PhyloSeqList_HMHZ_All.Rds and data/EimeriaSpeciesAssign.RDS
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### R/1_filtering.R
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Needs files data/PhyloSeqList_HMHZ_All.Rds and data/EimeriaSpeciesAssign.RDS
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quality filtering ASVs per amplicon and merging.
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final phyloseq object for further analysis is "tmp/PS.TSS_filtered.rds"
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R/2_dyadicMCMC_full.R --> Needs file "tmp/PS.TSS_filtered.rds"
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### R/2_dyadicMCMC_full.R
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Needs file "tmp/PS.TSS_filtered.rds"
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prepare dyadic data and do distance-based model for all the taxa (full model) with brms.
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model checks
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plotting figure 1
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### R/3_dyadicMCMC_groups.R
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R/3_dyadicMCMC_groups.R --> Needs file "tmp/PS.TSS_filtered.rds"
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Needs file "tmp/PS.TSS_filtered.rds"
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prepare data and do distance based model for parasites, plants, fungi and bacteria
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plotting figure 2 and 3 and 4ab
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R/4_Network.R --> Needs file "tmp/PS.TSS_filtered.rds"
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### R/4_Network.R
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Needs file "tmp/PS.TSS_filtered.rds"
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Co-occurrence network of Fungi, Parasites and Bacteria
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