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lbl08001.rst

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LBL08001 - Maintenance and Cleaning

Approved by: S. Thompson SOP No. LBL08001
Signed: Effective from:
Date: Last edited:

Purpose

Maintenance and Cleaning are fundamental to the operation of a safe and successful biolab. This document provides the daily, weekly and ad-hoc procedures that are in place.

Scope

  • This SOP applies to all users in all circumstances in the LBL biolab without exception.
  • In the case of specific instructions for Maintenance and Cleaning of equipment the user should defer to the relevant SOP for that particular piece of equipment.

Responsibilities

  • All users of the biolab are responsible for ensuring the clean and safe operation of equipment, lab furniture and tools.
  • The Biological Safety Officer has oversight and responsibility to make final decisions regarding maintenance and cleaning.

There are risks associated with certain cleaning products. Bleach is considered a hazardous substance and care must be taken to follow the instructions on the label.

It is the user's responsibility to check the MSDS for any chemical or cleaning products they are using.

Materials

The following items should be available in the lab

  • Disposable Nitrile gloves in various sizes
  • Plastic aprons
  • Eye protection
  • Plastic over-shoe protection
  • Cleaning products are available under the sink
  • A Mop and bucket
  • Blue roll
  • Disinfectant
  • Sterilizing Spray

Related documents

Definitions

BSO
Biological Safety Officer
SOP
Standard Operating Procedure

Procedures

Daily Maintenance and Cleaning procedures

Before starting work

  1. At the beginning of daily sessions the worktops should be cleared and any washing done and cleaned items returned to the shelves.
  2. Bins should be emptied.
  3. Any unlabelled liquid media should be emptied into the kill bin.
  4. Unknown/unlabelled and dirty glassware left out should be autoclaved, washed and returned to shelves.
  5. Work areas should be disinfected with Surgical spirit before work commences.

After Completing work

  1. Clear the work surfaces of equipment and consumables. Don't leave things out.
  2. All media and cultures should be labelled.
  3. Empty the general waste bins to the outside non-recyclables bin.
  4. Do the washing up.
  5. Make a record of the procedures and protocols in the lab day book.
  6. Ensure that electrical equipment is turned off, such as autoclave and RO pump

Weekly cleaning and maintenance procedures

  1. Mop the floor with a 10% bleach solution
  2. Empty the Kill bin of waste and refill with Virkon solution. See LBL04007
  3. Empty the EtBr waste bin if it is full. See
  4. Stock check the levels of consumables
    • Nitrile gloves
    • The spill kit
    • Bleach
    • Sterilizing Spray

Ad-Hoc cleaning and maintenance procedures

Disposal of equipment

Equipment that is disposed of, due to obsoletion or damage etc, will need to be cleaned and decontaminated if it has come into contact with GMO. This procedure must be assessed and carried out on a case by case basis.

Please contact the BSO if you plan to remove any equipment from the BioLab.

Resources

The SACGM Compendium of guidance Part 3: Containment and control of activities involving genetically modified microorganisms