description |
---|
Visualise cell positions at a glance |
To generate a heatmap of detected cells, which shows cell distributions in a more intuitive way that showing individual cell positions:
{% hint style="warning" %} This tool is a work in progress, and the usage may change {% endhint %}
heatmap cell_classification.xml heatmap.nii raw_data registered_atlas.nii -x 2 -y 2 -z 5
Run heatmap -h
to see all options.
- cellfinder classified cells file (usually
cell_classification.xml
) - Output filename. Should end with '.nii'. If the containing directory doesn't exist, it will be created.
- Path to raw data (just a single channel). Used to find the shape of the raw image that the detected cell positions are defined in.
- Registered atlas file from amap (typically run automatically in cellfinder). File is usually
registered_atlas.nii
.
{% hint style="warning" %} You must also specify the pixel sizes, see Specifying pixel size {% endhint %}
--heatmap-bin
Heatmap bin size (um of each edge of histogram cube)--heatmap-smoothing
Gaussian smoothing sigma, in um.--no-mask-figs
Don't mask the figures (removing any areas outside the brain, from e.g. smoothing)