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@@ -14,9 +14,9 @@ Although cellfinder is designed to be easy to install and use, if you're coming
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**The test dataset is large** \(~250GB\). It is recommended that you try this tutorial out on the fastest machine you have, with the fastest hard drive possible \(ideally SSD\) and an NVIDIA GPU. See [System requirements](../installation/system-requirements.md) for more details.
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{% endhint %}
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### Instructions
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## Instructions
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#### Setting up
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### Setting up
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* First install cellfinder, following the [Installation](../installation/installation.md) guide.
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* Download the data from [here](https://gin.g-node.org/cellfinder/data/raw/master/brainreg/test_brain.zip) \(it will take a long time to download\).
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* The orientation of your data. For atlas registration \(using [brainreg](https://docs.brainglobe.info/brainreg/introduction)\) the software needs to know how you acquired your data \(coronal, saggital etc.\). For this cellfinder uses [bg-space](https://github.com/brainglobe/bg-space). Full details on how to enter your data orientation can be found [here](https://docs.brainglobe.info/brainreg/user-guide#input-data-orientation), but for this tutorial, the orientation is `psl`, which means that the data origin is the most **p**osterior, **s**uperior, **l**eft voxel.
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* Which atlas you want to use \(you can see which are available by running `brainglobe list`. In this case, we want to use a mouse atlas \(as that's what our data is\), and we'll use the 10um version of the [Allen Mouse Brain Atlas](https://mouse.brain-map.org/static/atlas).
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#### Running cellfinder
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### Running cellfinder
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cellfinder runs with a single command, with various arguments that are detailed in [Command line options](../user-guide/usage/). To analyse the example data, the flags we need are:
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If your machine has less than 32GB of RAM, you should use the `allen_mouse_25um` atlas either way, as registration with the high-resolution atlas requires about 30GB for this image.
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{% endhint %}
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#### Visualising results
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### Inspecting the results
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#### Visualising cell detection performance
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cellfinder comes with a plugin for [napari](https://napari.org/) for easily visualising the results. To open napari, just run `napari` from your command line, and a viewer window should pop up.
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Into the window, then drag and drop:
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* The signal channel directory \(`test_brain/ch00`\)
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* The entire cellfinder output directory
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