Welcome to euf-specs Discussions! #1
eboileau
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Current specifications: EU format specifications (bedRMod)
For a rationale, see about EUF
What tools currently produce or handle MM/ML tags?
Oxford Nanopore Technology, e.g. Dorado (Remora, Bonito) write these tags to uBAMs. Modkit is a tool for working with modified bases, e.g. converting the information to bed-like format.
I believe Pacific Biosciences also uses read tags for per-read-base base modifications.
I have seen this, but I have not read the papers, nor tried the tools: modbamtools and modbedtools.
To facilitate access to and sharing of high-throughput transcriptome-wide RNA modification data, we need to harmonize data exchange formats. Our aim is to make data as FAIR as possible.
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