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nextflow.config
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// import profiles and workflow SHA from core
includeConfig "base.config"
// define workflow params
params {
help = false
version = false
fastq = null
bam = null
db_directory = null
threads = 4
host_reference = null
regions_bedfile = null
approx_size = 7000
assm_coverage = 60
trim_length = 150
prefix = null
primers = null
insert_reference = null
sample = null
sample_sheet = null
disable_ping = false
analyse_unclassified = false
override_basecaller_cfg = null
medaka_model_path = null
flye_quality = "nano-hq"
non_uniform_coverage = false
large_construct = false
full_reference = null
cutsite_mismatch = 1
primer_mismatch = 2
expected_coverage = 95
expected_identity = 99
assembly_tool = "flye"
canu_fast = false
client_fields = null
monochrome_logs = false
validate_params = true
show_hidden_params = false
schema_ignore_params = 'show_hidden_params,validate_params,monochrome_logs,aws_queue,aws_image_prefix,wf'
wf {
example_cmd = [
"--fastq 'wf-clone-validation-demo/fastq'",
"--primers 'wf-clone-validation-demo/primers.tsv'",
"--sample_sheet 'wf-clone-validation-demo/sample_sheet.csv'"
]
agent = null
}
}
manifest {
name = 'epi2me-labs/wf-clone-validation'
author = 'Oxford Nanopore Technologies'
homePage = 'https://github.com/epi2me-labs/wf-clone-validation'
description = 'De-novo reconstruction of synthetic plasmid sequences.'
mainScript = 'main.nf'
nextflowVersion = '>=23.04.2'
version = 'v1.7.3'
}
epi2melabs {
tags = "plasmid,denovo,annotation"
}