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fgsv should enable easier review in IGV #25

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tfenne opened this issue Sep 28, 2023 · 1 comment
Open

fgsv should enable easier review in IGV #25

tfenne opened this issue Sep 28, 2023 · 1 comment

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@tfenne
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tfenne commented Sep 28, 2023

fgsv generates an output BAM from the pileup phase that has tags like:

be:Z:0;left;from;read_pair
be:Z:0;left;into;read_pair
be:Z:0;left;from;split_read
be:Z:0;left;into;split_read
be:Z:0;right;from;split_read
be:Z:0;right;into;split_read

where records from the same template (or same read in the case of split reads) get different values. This makes it really hard to group/color/etc. in IGV and see all read alignments that support a given breakpoint.

I would suggest that some of this information should be extracted into individual tags. At least the breakpoint ID, and possibly also the left/right into a second tag. That way users could group by breakpoint ID and get a view of all the reads supporting the break point together.

@nh13
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nh13 commented Oct 25, 2023

@tfenne here's a little tool that may help for now: https://gist.github.com/nh13/79609e2f9d638e98e4db1a1b4a949b5e

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