The format is based on Keep a Changelog and this project adheres to Semantic Versioning.
- #106 - Added support for PacBio data
- Added
--taxon_reference
to be able to support both 'silva' and 'unite' - #157 - Added possibility to run double cutadapt steps,
--double_primer
- #211 - Added quality filter option
--maxEE
- #182 - Fix input in case there are no underscores in sample IDs
- #186 - Update github actions
- #187 - Sample ids are in incorrect order in feature-table from PacBio data
- #201 - Template update for nf-core/tools version 1.12.1
- #147 - Split
make_classifier
in two different processes that can be allocated different resources - #183 - Don't fetch taxonomy/create classifier when run with
--skip_taxonomy
- #180 - MultiQC, cutadapt and fastQC now work with
--multipleSequencingRuns
- Updated from cutadapt v2.6 to v2.8
- #170 - Cite paper for initial release
- #111 - Added parameter for user specified manifest file
- #118 - Added social preview images
- #135 - Added
--trunc_rmin
to make sure that auto trunc cutoff retaines a certain fraction of reads
- #172 - Template update for nf-core/tools v1.11
- #163 - Template update for nf-core/tools v1.10.2
- #136 - Pipeline fails with remote working directory
- #152 - Don't fetch taxonomy/create classifier when run with
--onlyDenoising
- Updated from MultiQC v1.6 to v1.9
--reads
is replaced by--input
due to nf-core/tools v1.10.2
- No further changes, except a bugfix for the timezone issue found by @marchoeppner
- Specification of
--qiime_timezone
might be required to run the analysis appropriately
- Update from QIIME2 v2018.6 to v2019.10, including DADA2 v1.6 to DADA2 v1.10
- Export absolute abundance files into 'results/abundance-table/filtered/' for optional external secondary analysis
- #78 - All sequenced classifed to the same species
- #40 - Added support for data originating from multiple sequencing runs
- #53 - DADA2 report is always exported
- #49 - Allowed more filtering options
- #5 - Introduced check for existence of input files
- Extended parameter sanity check, including #15
- #61 - Improved documentation
- #62 - Utilize nf-core/configs centrally for this pipeline
- #63 - QIIME imports files by using a manifest, giving more freedom with input file names
- #84 - Add proper nf-core logo
Initial release of nf-core/ampliseq, created with the nf-core template.