diff --git a/pandas-stubs/_typing.pyi b/pandas-stubs/_typing.pyi
index 635a418f..2655f09f 100644
--- a/pandas-stubs/_typing.pyi
+++ b/pandas-stubs/_typing.pyi
@@ -837,4 +837,9 @@ ExcelWriteEngine: TypeAlias = Literal["openpyxl", "odf", "xlsxwriter"]
 # https://github.com/pandas-dev/pandas-stubs/pull/1151#issuecomment-2715130190
 TimeZones: TypeAlias = str | tzinfo | None | int
 
+# Evaluates to a DataFrame column in DataFrame.assign context.
+IntoColumn: TypeAlias = (
+    AnyArrayLike | Scalar | Callable[[DataFrame], AnyArrayLike | Scalar]
+)
+
 __all__ = ["npt", "type_t"]
diff --git a/pandas-stubs/core/frame.pyi b/pandas-stubs/core/frame.pyi
index 11449493..f88bd0c6 100644
--- a/pandas-stubs/core/frame.pyi
+++ b/pandas-stubs/core/frame.pyi
@@ -100,6 +100,7 @@ from pandas._typing import (
     InterpolateOptions,
     IntervalClosedType,
     IntervalT,
+    IntoColumn,
     JoinHow,
     JsonFrameOrient,
     Label,
@@ -728,7 +729,7 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
         value: Scalar | ListLikeU | None,
         allow_duplicates: _bool = ...,
     ) -> None: ...
-    def assign(self, **kwargs) -> Self: ...
+    def assign(self, **kwargs: IntoColumn) -> Self: ...
     def align(
         self,
         other: NDFrameT,
diff --git a/tests/test_frame.py b/tests/test_frame.py
index eca3cec8..99f11b3c 100644
--- a/tests/test_frame.py
+++ b/tests/test_frame.py
@@ -308,6 +308,38 @@ def test_types_assign() -> None:
     df["col3"] = df.sum(axis=1)
 
 
+def test_assign() -> None:
+    df = pd.DataFrame({"a": [1, 2, 3], 1: [4, 5, 6]})
+
+    my_unnamed_func = lambda df: df["a"] * 2
+
+    def my_named_func_1(df: pd.DataFrame) -> pd.Series[str]:
+        return df["a"]
+
+    def my_named_func_2(df: pd.DataFrame) -> pd.Series[Any]:
+        return df["a"]
+
+    check(assert_type(df.assign(c=lambda df: df["a"] * 2), pd.DataFrame), pd.DataFrame)
+    check(
+        assert_type(df.assign(c=lambda df: df["a"].index), pd.DataFrame), pd.DataFrame
+    )
+    check(
+        assert_type(df.assign(c=lambda df: df["a"].to_numpy()), pd.DataFrame),
+        pd.DataFrame,
+    )
+    check(
+        assert_type(df.assign(c=lambda df: df["a"].max()), pd.DataFrame),
+        pd.DataFrame,
+    )
+    check(assert_type(df.assign(c=df["a"] * 2), pd.DataFrame), pd.DataFrame)
+    check(assert_type(df.assign(c=df["a"].index), pd.DataFrame), pd.DataFrame)
+    check(assert_type(df.assign(c=df["a"].to_numpy()), pd.DataFrame), pd.DataFrame)
+    check(assert_type(df.assign(c=2), pd.DataFrame), pd.DataFrame)
+    check(assert_type(df.assign(c=my_unnamed_func), pd.DataFrame), pd.DataFrame)
+    check(assert_type(df.assign(c=my_named_func_1), pd.DataFrame), pd.DataFrame)
+    check(assert_type(df.assign(c=my_named_func_2), pd.DataFrame), pd.DataFrame)
+
+
 def test_types_sample() -> None:
     df = pd.DataFrame(data={"col1": [1, 2], "col2": [3, 4]})
     # GH 67