diff --git a/.github/workflows/master_e2e.yaml b/.github/workflows/master_e2e.yaml
index 7e1be31c7..9b18d199b 100644
--- a/.github/workflows/master_e2e.yaml
+++ b/.github/workflows/master_e2e.yaml
@@ -9,8 +9,8 @@ on:
         description: Version of backup-restore-operator to use
         type: string
       boot_type:
-        description: Choose booting type (pxe, iso, raw) 
-        default: pxe 
+        description: Choose booting type (pxe, iso, raw)
+        default: pxe
         type: string
       ca_type:
         description: CA type to use (selfsigned or private)
diff --git a/tests/assets/osChannel.yaml b/tests/assets/osChannel.yaml
new file mode 100644
index 000000000..8dc633969
--- /dev/null
+++ b/tests/assets/osChannel.yaml
@@ -0,0 +1,11 @@
+apiVersion: elemental.cattle.io/v1beta1
+kind: ManagedOSVersionChannel
+metadata:
+  name: os-channel-to-test
+  # namespace: fleet-default
+spec:
+  deleteNoLongerInSyncVersions: true
+  options:
+    image: %OS_CHANNEL%
+  syncInterval: 1h
+  type: custom
diff --git a/tests/e2e/seedImage_test.go b/tests/e2e/seedImage_test.go
index 405e3cc64..e1da72f9b 100644
--- a/tests/e2e/seedImage_test.go
+++ b/tests/e2e/seedImage_test.go
@@ -18,6 +18,7 @@ import (
 	"os"
 	"os/exec"
 	"strconv"
+	"strings"
 	"time"
 
 	. "github.com/onsi/ginkgo/v2"
@@ -42,6 +43,62 @@ var _ = Describe("E2E - Creating ISO image", Label("iso-image"), func() {
 		// Report to Qase
 		testCaseID = 38
 
+		// If registry keyword is found this means that we have to test a specific OS channel
+		if strings.Contains(os2Test, "registry") {
+			// Get default channel image
+			defChannel, err := kubectl.RunWithoutErr("get", "managedOSVersionChannel",
+				"--namespace", clusterNS,
+				"-o", "jsonpath={.items[0].spec.options.image}")
+			Expect(err).To(Not(HaveOccurred()))
+			Expect(defChannel).To(Not(BeEmpty()))
+
+			// Add channel to test if needed
+			if !strings.Contains(defChannel, os2Test) {
+				By("Adding OSChannel to test", func() {
+					// Get channel name (to be able to remove it later)
+					channelName, err := kubectl.RunWithoutErr("get", "managedOSVersionChannel",
+						"--namespace", clusterNS,
+						"-o", "jsonpath={.items[0].metadata.name}")
+					Expect(err).To(Not(HaveOccurred()))
+					Expect(channelName).To(Not(BeEmpty()))
+
+					// Set temporary file
+					osChannelTmp, err := tools.CreateTemp("osChannel")
+					Expect(err).To(Not(HaveOccurred()))
+					defer os.Remove(osChannelTmp)
+
+					// Save original file as it can be modified multiple time
+					err = tools.CopyFile(osChannelYaml, osChannelTmp)
+					Expect(err).To(Not(HaveOccurred()))
+
+					// Set the OS channel to test
+					err = tools.Sed("%OS_CHANNEL%", os2Test, osChannelTmp)
+					Expect(err).To(Not(HaveOccurred()))
+
+					// Apply to k8s
+					err = kubectl.Apply(clusterNS, osChannelTmp)
+					Expect(err).To(Not(HaveOccurred()))
+
+					// Check that the OS channel to test has been added
+					const channel = "os-channel-to-test"
+					newChannel, err := kubectl.RunWithoutErr("get", "managedOSVersionChannel",
+						"--namespace", clusterNS, channel,
+						"-o", "jsonpath={.spec.options.image}")
+					Expect(err).To(Not(HaveOccurred()))
+					Expect(newChannel).To(Equal(os2Test))
+
+					// Delete the default channel (to be sure that it can't be used)
+					out, err := kubectl.RunWithoutErr("delete", "managedOSVersionChannel",
+						"--namespace", clusterNS, channelName)
+					Expect(err).To(Not(HaveOccurred()))
+					Expect(out).To(ContainSubstring("deleted"))
+				})
+			}
+
+			// Clear OS_TO_TEST, as Staging and Dev channels manually added do not contain "unstable" tag
+			os2Test = ""
+		}
+
 		By("Adding SeedImage", func() {
 			var (
 				baseImageURL string
diff --git a/tests/e2e/suite_test.go b/tests/e2e/suite_test.go
index 296cd18e6..a48dfea8a 100644
--- a/tests/e2e/suite_test.go
+++ b/tests/e2e/suite_test.go
@@ -49,6 +49,7 @@ const (
 	localStorageYaml      = "../assets/local-storage.yaml"
 	metallbRscYaml        = "../assets/metallb_rsc.yaml"
 	numberOfNodesMax      = 30
+	osChannelYaml         = "../assets/osChannel.yaml"
 	resetMachineInv       = "../assets/reset_machine_inventory.yaml"
 	restoreYaml           = "../assets/restore.yaml"
 	sshConfigFile         = "../assets/ssh_config"