diff --git a/ot2_moclo_jove/miniprep/data/miniprep_template.py b/ot2_moclo_jove/miniprep/data/miniprep_template.py index 85452b2..52b9bdc 100644 --- a/ot2_moclo_jove/miniprep/data/miniprep_template.py +++ b/ot2_moclo_jove/miniprep/data/miniprep_template.py @@ -16,22 +16,22 @@ spm = [buffers.wells(8), buffers.wells(9)] eb = buffers.wells(10) -#Add 500 無 ETR and 20 無 Mag-Bind -p300.transfer(520, etr_mag.bottom(0.5), +#Add 500 繕L ETR and 20 繕L Mag-Bind +p300.transfer(520, etr_mag.bottom(0.5)) #Wait 5 min #Magnetize and discard supernatant -#Demagnetize and add 500 無 ETR +#Demagnetize and add 500 繕L ETR #Magnetize and discard supernatant -#Demagnetize and add 700 無 VHB +#Demagnetize and add 700 繕L VHB #Magnetize and discard supernatant -#Demagnetize and add 700 無 VHB +#Demagnetize and add 700 繕L VHB #Magnetize and discard supernatant -#Demagnetize and aAdd 700 無 SPM +#Demagnetize and aAdd 700 繕L SPM #Magnetize and discard supernatant #Wait 1 min #Discard last bit of supernatant #Wait 9 min -#Demagnetize and add 50-100 無 Elution Buffer (might be able to add less) +#Demagnetize and add 50-100 繕L Elution Buffer (might be able to add less) #Magnetize and remove and save supernatant (which contains dna)