From 822450800b8dc42e5ee9e5a8d759be6541e9ba26 Mon Sep 17 00:00:00 2001 From: Benjamin Chan Date: Thu, 1 Aug 2019 16:18:23 -0400 Subject: [PATCH 1/2] Deleted a stray comma and added a closing parenthesis --- ot2_moclo_jove/miniprep/data/miniprep_template.py | 14 +++++++------- 1 file changed, 7 insertions(+), 7 deletions(-) diff --git a/ot2_moclo_jove/miniprep/data/miniprep_template.py b/ot2_moclo_jove/miniprep/data/miniprep_template.py index 85452b2..43db720 100644 --- a/ot2_moclo_jove/miniprep/data/miniprep_template.py +++ b/ot2_moclo_jove/miniprep/data/miniprep_template.py @@ -16,22 +16,22 @@ spm = [buffers.wells(8), buffers.wells(9)] eb = buffers.wells(10) -#Add 500 µL ETR and 20 µL Mag-Bind -p300.transfer(520, etr_mag.bottom(0.5), +#Add 500 �L ETR and 20 �L Mag-Bind +p300.transfer(520, etr_mag.bottom(0.5)) #Wait 5 min #Magnetize and discard supernatant -#Demagnetize and add 500 µL ETR +#Demagnetize and add 500 �L ETR #Magnetize and discard supernatant -#Demagnetize and add 700 µL VHB +#Demagnetize and add 700 �L VHB #Magnetize and discard supernatant -#Demagnetize and add 700 µL VHB +#Demagnetize and add 700 �L VHB #Magnetize and discard supernatant -#Demagnetize and aAdd 700 µL SPM +#Demagnetize and aAdd 700 �L SPM #Magnetize and discard supernatant #Wait 1 min #Discard last bit of supernatant #Wait 9 min -#Demagnetize and add 50-100 µL Elution Buffer (might be able to add less) +#Demagnetize and add 50-100 �L Elution Buffer (might be able to add less) #Magnetize and remove and save supernatant (which contains dna) From 18a8a752401a76d82c68c727cb327f5b5853fd25 Mon Sep 17 00:00:00 2001 From: Benjamin Chan Date: Thu, 1 Aug 2019 16:20:17 -0400 Subject: [PATCH 2/2] =?UTF-8?q?Fixed=20the=20=C2=B5L=20labels?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- ot2_moclo_jove/miniprep/data/miniprep_template.py | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/ot2_moclo_jove/miniprep/data/miniprep_template.py b/ot2_moclo_jove/miniprep/data/miniprep_template.py index 43db720..52b9bdc 100644 --- a/ot2_moclo_jove/miniprep/data/miniprep_template.py +++ b/ot2_moclo_jove/miniprep/data/miniprep_template.py @@ -16,22 +16,22 @@ spm = [buffers.wells(8), buffers.wells(9)] eb = buffers.wells(10) -#Add 500 �L ETR and 20 �L Mag-Bind +#Add 500 µL ETR and 20 µL Mag-Bind p300.transfer(520, etr_mag.bottom(0.5)) #Wait 5 min #Magnetize and discard supernatant -#Demagnetize and add 500 �L ETR +#Demagnetize and add 500 µL ETR #Magnetize and discard supernatant -#Demagnetize and add 700 �L VHB +#Demagnetize and add 700 µL VHB #Magnetize and discard supernatant -#Demagnetize and add 700 �L VHB +#Demagnetize and add 700 µL VHB #Magnetize and discard supernatant -#Demagnetize and aAdd 700 �L SPM +#Demagnetize and aAdd 700 µL SPM #Magnetize and discard supernatant #Wait 1 min #Discard last bit of supernatant #Wait 9 min -#Demagnetize and add 50-100 �L Elution Buffer (might be able to add less) +#Demagnetize and add 50-100 µL Elution Buffer (might be able to add less) #Magnetize and remove and save supernatant (which contains dna)