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This might include some personal or unpublished info.</div><a class="article-more button is-small is-size-7" href="/2025/02/10/%E7%A4%BE%E4%BA%A4%E4%B8%AD%E7%9A%84%E6%80%A7%E5%88%AB%E5%81%8F%E8%A7%81%E5%8F%8A%E5%85%B6%E8%A1%A8%E5%BE%81%E6%89%8B%E6%AE%B5/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-06-30T00:05:04.000Z" title="2023-06-30T00:05:04.000Z">2023-06-29</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-06-30T00:08:30.000Z" title="2023-06-30T00:08:30.000Z">2023-06-29</time></span><span class="level-item"><a class="link-muted" href="/categories/Notes/">Notes</a><span> / </span><a class="link-muted" href="/categories/Notes/Experiment/">Experiment</a></span><span class="level-item">a minute read (About 153 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/06/29/Exp-Culture-Medium-Preparation/">[Exp]Culture Medium Preparation</a></h1><div class="content">This article is locked. This might include some personal or unpublished info.</div><a class="article-more button is-small is-size-7" href="/2023/06/29/Exp-Culture-Medium-Preparation/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-06-28T20:49:32.000Z" title="2023-06-28T20:49:32.000Z">2023-06-28</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-07-12T21:37:34.514Z" title="2023-07-12T21:37:34.514Z">2023-07-12</time></span><span class="level-item"><a class="link-muted" href="/categories/Refs/">Refs</a><span> / </span><a class="link-muted" href="/categories/Refs/Enzyme/">Enzyme</a></span><span class="level-item">3 minutes read (About 464 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/06/28/RefNote-The-evolution-of-multiple-active-site-configurations-in-a-designed-enzyme/">RefNote: The evolution of multiple active site configurations in a designed enzyme KE07 &amp; Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series</a></h1><div class="content"><blockquote>
<p>Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series<br><a target="_blank" rel="noopener" href="https://www.sciencedirect.com/science/article/pii/S0022283609015381?via%3Dihub">https://www.sciencedirect.com/science/article/pii/S0022283609015381?via%3Dihub</a></p>
</blockquote>
<blockquote>
<p>The evolution of multiple active site configurations in a designed enzyme<br><a target="_blank" rel="noopener" href="https://www.nature.com/articles/s41467-018-06305-y">https://www.nature.com/articles/s41467-018-06305-y</a></p>
</blockquote>
<ul>
<li>Important experimental protocol reference (background reaction; Arrhenius kinetics)</div><a class="article-more button is-small is-size-7" href="/2023/06/28/RefNote-The-evolution-of-multiple-active-site-configurations-in-a-designed-enzyme/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-05-24T22:48:25.000Z" title="2023-05-24T22:48:25.000Z">2023-05-24</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-05-25T00:02:47.000Z" title="2023-05-25T00:02:47.000Z">2023-05-24</time></span><span class="level-item"><a class="link-muted" href="/categories/Refs/">Refs</a><span> / </span><a class="link-muted" href="/categories/Refs/Enzyme/">Enzyme</a></span><span class="level-item">2 minutes read (About 352 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/05/24/RefNote-Electric-Field-Design%E5%90%88%E9%9B%86/">RefNote-Electric-Field-Design合集</a></h1><div class="content">This article is locked. This might include some personal or unpublished info.</div><a class="article-more button is-small is-size-7" href="/2023/05/24/RefNote-Electric-Field-Design%E5%90%88%E9%9B%86/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-05-08T21:02:28.000Z" title="2023-05-08T21:02:28.000Z">2023-05-08</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-05-08T22:24:18.837Z" title="2023-05-08T22:24:18.837Z">2023-05-08</time></span><span class="level-item"><a class="link-muted" href="/categories/Notes/">Notes</a><span> / </span><a class="link-muted" href="/categories/Notes/Tools/">Tools</a></span><span class="level-item">a few seconds read (About 104 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/05/08/HMMER-Notes/">HMMER Notes</a></h1><div class="content"><p>HMMer notes, avoid redundant searching</p></div><a class="article-more button is-small is-size-7" href="/2023/05/08/HMMER-Notes/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-04-15T03:18:36.000Z" title="2023-04-15T03:18:36.000Z">2023-04-14</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-04-15T03:19:54.415Z" title="2023-04-15T03:19:54.415Z">2023-04-14</time></span><span class="level-item"><a class="link-muted" href="/categories/Refs/">Refs</a><span> / </span><a class="link-muted" href="/categories/Refs/Enzyme/">Enzyme</a></span><span class="level-item">a few seconds read (About 109 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/04/14/RefNote-AsiteDesign/">RefNote: AsiteDesign</a></h1><div class="content">This article is locked. This might include some personal or unpublished info.</div><a class="article-more button is-small is-size-7" href="/2023/04/14/RefNote-AsiteDesign/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-04-15T02:56:52.000Z" title="2023-04-15T02:56:52.000Z">2023-04-14</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-04-15T03:00:03.813Z" title="2023-04-15T03:00:03.813Z">2023-04-14</time></span><span class="level-item"><a class="link-muted" href="/categories/Refs/">Refs</a><span> / </span><a class="link-muted" href="/categories/Refs/Enzyme/">Enzyme</a></span><span class="level-item">2 minutes read (About 325 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/04/14/RefNote-Using-High-Throughput-Molecular-Dynamics-Simulation-to-Enhance-the-Computational-Design-of-Kemp-Elimination-Enzymes/">RefNote: Using High-Throughput Molecular Dynamics Simulation to Enhance the Computational Design of Kemp Elimination Enzymes</a></h1><div class="content">This article is locked. This might include some personal or unpublished info.</div><a class="article-more button is-small is-size-7" href="/2023/04/14/RefNote-Using-High-Throughput-Molecular-Dynamics-Simulation-to-Enhance-the-Computational-Design-of-Kemp-Elimination-Enzymes/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-03-09T23:51:55.000Z" title="2023-03-09T23:51:55.000Z">2023-03-09</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-09-01T23:49:29.004Z" title="2023-09-01T23:49:29.004Z">2023-09-01</time></span><span class="level-item"><a class="link-muted" href="/categories/Notes/">Notes</a><span> / </span><a class="link-muted" href="/categories/Notes/Experiment/">Experiment</a></span><span class="level-item">a minute read (About 120 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/03/09/Exp-Kinetic-Assay-KE/">[Exp] Kinetic Assay KE</a></h1><div class="content">This article is locked. This might include some personal or unpublished info.</div><a class="article-more button is-small is-size-7" href="/2023/03/09/Exp-Kinetic-Assay-KE/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-02-01T20:45:53.000Z" title="2023-02-01T20:45:53.000Z">2023-02-01</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-04-15T02:54:34.463Z" title="2023-04-15T02:54:34.463Z">2023-04-14</time></span><span class="level-item"><a class="link-muted" href="/categories/Refs/">Refs</a><span> / </span><a class="link-muted" href="/categories/Refs/code-O/">code-O</a></span><span class="level-item">a few seconds read (About 30 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/02/01/RefNote-code-O/">RefNote: mechanisms of oxygen resistance of enzyme</a></h1><div class="content">This article is locked. This might include some personal or unpublished info.</div><a class="article-more button is-small is-size-7" href="/2023/02/01/RefNote-code-O/#more">Read more</a></article></div><div class="card"><article class="card-content article" role="article"><div class="article-meta is-size-7 is-uppercase level is-mobile"><div class="level-left"><span class="level-item">Posted&nbsp;<time dateTime="2023-01-19T22:41:51.000Z" title="2023-01-19T22:41:51.000Z">2023-01-19</time></span><span class="level-item">Updated&nbsp;<time dateTime="2023-06-30T00:03:49.000Z" title="2023-06-30T00:03:49.000Z">2023-06-29</time></span><span class="level-item"><a class="link-muted" href="/categories/Notes/">Notes</a><span> / </span><a class="link-muted" href="/categories/Notes/Experiment/">Experiment</a></span><span class="level-item">3 minutes read (About 444 words)</span></div></div><h1 class="title is-3 is-size-4-mobile"><a class="link-muted" href="/2023/01/19/Exp-Sterilization/">[Exp]Sterilization</a></h1><div class="content">This article is locked. This might include some personal or unpublished info.</div><a class="article-more button is-small is-size-7" href="/2023/01/19/Exp-Sterilization/#more">Read more</a></article></div><nav class="pagination" role="navigation" aria-label="pagination"><div class="pagination-previous is-invisible is-hidden-mobile"><a href="/page/0/">Previous</a></div><div class="pagination-next"><a href="/page/2/">Next</a></div><ul class="pagination-list is-hidden-mobile"><li><a class="pagination-link is-current" href="/">1</a></li><li><a class="pagination-link" href="/page/2/">2</a></li><li><span class="pagination-ellipsis">&hellip;</span></li><li><a class="pagination-link" href="/page/7/">7</a></li></ul></nav></div><div class="column column-left is-4-tablet is-4-desktop is-3-widescreen  order-1 is-sticky"><div class="card widget" data-type="profile"><div class="card-content"><nav class="level"><div class="level-item has-text-centered flex-shrink-1"><div><figure class="image is-128x128 mx-auto mb-2"><img class="avatar" src="/img/avatar.png" alt="Q.Z. 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