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README.md

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@@ -93,6 +93,6 @@ To run the code, you could then copy and paste the code from the workshop vignet
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## Instructor names and contact information
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* Maria Doyle <Maria.Doyle at petermac.org>
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* Stefano Mangiola <mangiola.s at wehi.edu.au>
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* Maria Doyle <Maria.Doyle at petermac.org>
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vignettes/tidytranscriptomics_case_study.Rmd

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@@ -150,14 +150,14 @@ We can use `select` to view columns, for example, to see the filename, total cel
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```{r}
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sce_obj |> select(file, nCount_RNA, Phase)
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```
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A couple of things to note with `select`
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> As we did not output the .cell column we get a tibble instead of a SingleCellExperiment object and a message to let us know: "tidySingleCellExperiment says: Key columns are missing. A data frame is returned for independent data analysis." This is ok as it's what we want here when exploring the data.
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* As we did not output the .cell column we get a tibble instead of a SingleCellExperiment object and a message to let us know: `tidySingleCellExperiment says: Key columns are missing. A data frame is returned for independent data analysis.` This is ok as it's what we want here when viewing the columns.
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> If we use select to output the .cell (key) column we will also get any view-only columns returned, such as the UMAP columns generated during the preprocessing.
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>```{r}
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> sce_obj |> select(.cell, nCount_RNA, Phase)
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>```
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* If we use `select` to output the .cell (key) column we will also get any view-only columns returned, such as the UMAP columns generated during the preprocessing.
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```{r}
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sce_obj |> select(.cell, nCount_RNA, Phase)
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```
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We can use `mutate` to create a column. For example, we could create a new `Phase_l` column that contains a lower-case version of `Phase`.
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Using the `sce_obj`:
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1. What proportion of all cells are gamma-delta T cells? Use signature_score > 0.7 to identify gamma-delta T cells.
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1. What proportion of all cells are gamma-delta T cells? Use signature_score > 0.7 to identify gamma-delta T cells.
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2. There is a cluster of cells characterised by a low RNA output (nCount_RNA < 100). Identify the cell composition (cell_type) of that cluster.
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2. There is a cluster of cells characterised by a low RNA output (nCount_RNA < 100). Identify the cell composition (cell_type) of that cluster.
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# Pseudobulk analyses
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