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3' end clipping not working #14

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donutbrew opened this issue Jul 7, 2020 · 1 comment
Open

3' end clipping not working #14

donutbrew opened this issue Jul 7, 2020 · 1 comment

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@donutbrew
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Hi there--I have noticed that 3' ends are not clipped. I've played around a little with the -u and -d flags, but nothing seems to really change what is going on with the 3' ends of the reads. Below, I am pasting an IGV plot of what I mean--you can see in the clipped vs unclipped, the primer coverage drops by half--those are only those reads that ahve a primer on the 5' end. Am I missing something? (very possible!) I do need to get both primers clipped, so if I am missing a setting somewhere, please let me know.

image

@arz19893
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got the same issue, any tips ?

Thanks (:

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