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After installing pyinstaller, run the following command to create a single executable
64 Bit pyinstaller -F --add-data c:\windows\system32\libusb-1.0.dll;. WinBusKill.py
pyinstaller -F --add-data c:\windows\system32\libusb-1.0.dll;. WinBusKill.py
32 bit pyinstaller -F --add-data c:\windows\SysWOW64\libusb-1.0.dll;. WinBusKill.py
pyinstaller -F --add-data c:\windows\SysWOW64\libusb-1.0.dll;. WinBusKill.py
You will now have a windows executable which can be run.