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dnanexus/qckiller.py
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chip-seq-pipeline
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==========
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ENCODE ChIP-seq Pipeline
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==========
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ENCODE Uniform processing pipeline for ChIP-seq
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===============================================
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Current implementation is deployed to the DNAnexus platform.
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Mapping
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-------
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1. Map reads with BWA, mark duplicates Picard, and remove duplicates.
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2. Estimate library complexity and calculate calculate NRF (non-redundant fraction), PBC1, PBC2 (PCR bottleneck coefficient).
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3. Calculate cross-correlation analysis with spp/phantompeakqualtools.
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4. Generate p-value and fold-over-control signal tracks for each replicate and replicates pooled with MACS2.
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Peak calling (histone marks)
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----------------------------
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1. Call peaks with MACS2.
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2. Calculate and report overlapping peaks from both replicates.
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Peak calling (transcription factors)
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------------------------------------
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1. Call peaks with SPP.
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2. Threshold peaks with IDR.
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3. Report IDR-thresholded peak sets, self-consistency ratio, rescue ratio, reproducibility test.

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