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Specify the location of the BED files available for filtering
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maude-ardin committed Oct 25, 2016
1 parent 539abaf commit fc2802f
Showing 1 changed file with 11 additions and 4 deletions.
15 changes: 11 additions & 4 deletions mutspecFilter.xml
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<param name="segDup" type="boolean" checked="true" truevalue="--segDup" falsevalue="" label="Filter against SegDup database" help="Remove variants present at &#62;= 0.9 frequency in the genomic duplicate segments database" />
<param name="esp" type="boolean" checked="true" truevalue="--esp" falsevalue="" label="Filter against the ESP database" help="Remove variants present at frequency &#62; 0.001 in the Exome Sequencing Project database (only valid for human genomes)" />
<param name="thG" type="boolean" checked="true" truevalue="--thG" falsevalue="" label="Filter against the 1000g database project" help="Remove variants present at frequency &#62; 0.001 in the 1000 genome database (only valid for human genomes)" />
<param name="exac" type="boolean" checked="true" truevalue="--exac" falsevalue="" label="Filter against the ExAC database" help="Remove variants present at frequency &#62; 0.001 in the EXome Agregate Consortium database" />
<param name="exac" type="boolean" checked="true" truevalue="--exac" falsevalue="" label="Filter against the ExAC database" help="Remove variants present at frequency &#62; 0.001 in the EXome Agregate Consortium database (only valid for human genomes)" />

<repeat name="filters" title="Additional filters">
<param name="reference" type="data" format="bed" label="bed file reference" help="filter regions"/>
<param name="reference" type="data" format="bed" label="Reference file (bed or vcf)" help="Remove variants present in the reference file"/>
</repeat>

</inputs>
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**What it does**

Filter a file annotated with MutSpec-Annot tool. Variants present in public databases (dbSNP, SegDup, ESP, 1000 genome obtained from Annovar) will be removed from the input file (with frequency limits described above).
Filter a file annotated with MutSpec-Annot tool. Variants present in public databases obtained from Annovar will be removed from the input file (with frequency limits described above).

.. class:: warningmark

The databases ESP and 1000 genome can be used only for human genomes
The databases ESP, 1000 genome and ExAC can be used only for human genomes

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You eventually would like to filter for additional features like repeats and tandem repeats.
You just need to provide the reference in vcf or bed format.

.. class:: infomark

Reference files are available on IARC Galaxy Shared Data.
On the top panel click on "Shared Data" and select "Data Libraries".
The category "BED annotations" contains reference files for different genomes.


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**Output**
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