v2.4.0 - More representative BigWig output
Previous versions of the bamToBw.pl
script included all reads in the input BAM/CRAM in the counts. Historically all of the common tools for generating these files have done this. With the introduction of several new flags for BWA-mem the data becomes skewed (particularly by supplimentary reads).
This release filters reads where the flags overlap with 3844
- 4 - read unmapped
- 256 - Not primary alignment
- 512 - read fails platform/vendor quality checks
- 1024 - read is PCR or optical duplicate
- 2048 - supplementary alignment
See: explain-flags
We plan to expose the parameter in the future so please add support to the issue #121 if you want to be able to control this value.