Adding spherized profiles

README.md

@@ -1,25 +1,29 @@
-# Processed Data for the LINCS Cell Painting Project
+# LINCS Cell Painting profile data repository
 
-The repository stores data and data processing scripts for **a subset** of the [Broad Drug Repurposing Hub](https://clue.io/repurposing#home) collection of compounds.
+The Library of Integrated Network-Based Cellular Signatures (LINCS) Project aims to create publicly available resources to characterize how cells respond to perturbation.
+This repository stores Cell Painting readouts and associated data-processing pipelines for the LINCS Cell Painting dataset.
 
-In this project, the [Connectivity Map](https://clue.io/team) team perturbed A549 cells with ~1,500 compounds across 6 doses in 5 technical replicates. 
+The data represent **a subset** of the [Broad Drug Repurposing Hub](https://clue.io/repurposing#home) collection of compounds.
+
+In this project, the [Connectivity Map](https://clue.io/team) team perturbed A549 cells with ~1,500 compounds across 6 doses in 5 technical replicates.
 We refer to this dataset as `LINCS Pilot 1`.
+We also include data for the second batch of LINCS Cell Painting data, which we refer to as `LKCP`.
 
-For a specific list of compounds tested, see [`metadata`](https://github.com/broadinstitute/lincs-cell-painting/tree/master/metadata). 
-Information about the compounds can be interactively explored in the [CLUE Repurposing app](https://clue.io/repurposing-app). 
+For a specific list of compounds tested, see [`metadata`](https://github.com/broadinstitute/lincs-cell-painting/tree/master/metadata).
+You can interactively explore information about the compounds in the [CLUE Repurposing app](https://clue.io/repurposing-app).
 The [Morphology Connectivity Hub](https://clue.io/morphology) is the primary source of this dataset.
 
-## Image-Based Profiling
+## Image-Based profiling
 
-We apply a unified, image-based profiling pipeline to all 136 384-well plates from `LINCS Pilot 1` .
+We apply a unified, image-based profiling pipeline to all 136 384-well plates from `LINCS Pilot 1`, and all 135 384-well plates from `LKCP`.
 We use [pycytominer](https://github.com/cytomining/pycytominer) as the primary tool for image-based profiling.
 
-The profiles are processed and stored in the [profiles/](profiles/) directory.
+We process and store profiles in the [profiles/](profiles/) directory.
 See [`profiles/README.md`](profiles/README.md) for more details and for instructions on how to reproduce the pipeline.
 
 For more details about image-based profiling in general, please refer to [Caicedo et al. 2017](https://doi.org/10.1038/nmeth.4397).
 
-## Computational Environment
+## Computational environment
 
 We use [conda](https://docs.conda.io/en/latest/) to manage the computational environment.
 

profiles/README.md

@@ -1,8 +1,9 @@
-# Image-Based Profiling
+# Image-Based profiling
 
 Image-based profiling represents a series of data processing steps that turn image-based readouts into more manageable data matrices for downstream analyses ([Caicedo et al. 2017](https://doi.org/10.1038/nmeth.4397)).
-Typically, the image-based readouts are derived from CellProfiler ([McQuin et al. 2018](https://doi.org/10.1371/journal.pbio.2005970)) and represent single cell morphology measurements.
-In this folder, we process the CellProfiler derived morphology features using [pycytominer](https://github.com/cytomining/pycytominer) - a tool enabling reproducible image-based profiling.
+Typically, you derive image-based readouts using software, like CellProfiler ([McQuin et al. 2018](https://doi.org/10.1371/journal.pbio.2005970)), that segment cells and extract so-called hand-engineered single cell morphology measurements.
+In this folder, we process the CellProfiler derived morphology features for the LINCS Cell Painting dataset using [pycytominer](https://github.com/cytomining/pycytominer) - a tool enabling reproducible image-based profiling.
+
 Specifically, we include:
 
 1. Data processing scripts to perform the full unified, image-based profiling pipeline
@@ -11,34 +12,52 @@ Specifically, we include:
 
 ## Workflow
 
-![Cytominer Workflow](media/cytominer_workflow.png)
+![Cytominer workflow](media/cytominer_workflow.png)
 
 Note here that we do not include the intermediate step of generating `.sqlite` files per plate using a tool called [cytominer-database](https://github.com/cytomining/cytominer-database).
 This repository and workflow begins after we applied cytominer-database.
 
 ## Data Levels
 
-### CellProfilier-derived Profiles
+We include two batches of Cell Painting data in this repository: `2016_04_01_a549_48hr_batch1` and `2017_12_05_Batch2`.
+
+### CellProfilier-derived profiles
+
+For each batch, we include:
 
-| Data Level | Description | File Format | Included in this Repo |
+| Data level | Description | File format | Included in this repo? |
 | :--------- | :---------- | :---------- | :-------------------- |
-| Level 1 | Cell Images | `.tif` | No^ |
-| Level 2 | Single Cell Profiles | `.sqlite` | No^ |
-| Level 3 | Aggregated Profiles with Metadata | `.csv.gz` | Yes |
-| Level 4a | Normalized Profiles with Metadata | `.csv.gz` | Yes |
-| Level 4b | Normalized and Feature Selected Profiles with Metadata | `.csv.gz` | Yes |
-| Level 5 | Consensus Perturbation Profiles | `.csv.gz` | Yes |
+| Level 1 | Cell images | `.tif` | No^ |
+| Level 2 | Single cell profiles | `.sqlite` | No^ |
+| Level 3 | Aggregated profiles with metadata | `.csv.gz` | Yes |
+| Level 4a | Normalized profiles with metadata | `.csv.gz` | Yes |
+| Level 4b | Normalized and feature selected profiles with metadata | `.csv.gz` | Yes |
+| Level 5 | Consensus perturbation profiles | `.csv.gz` | Yes |
 
 Importantly, we include files for _two_ different types of normalization: Whole-plate normalization, and DMSO-specific normalization.
-See [`profile.py`](profile.py) for more details.
+See [`profile_cells.py`](profile_cells.py) for more details.
+
+#### Batch corrected profiles
+
+We use a spherize (a.k.a. whiten) transform to adjust for plate position effects.
+The spherize transform adjusts for plate position effects by transforming the profile data such that the DMSO profiles are left with an identity covariance matrix.
+See [`spherize-batch-effects.ipynb`](spherized_profiles/spherize-batch-effects.ipynb) for implementation details.
+
+For each batch we include four different spherized profiles.
+These data include all level 4b profiles for every batch.
 
-^ Note that these files are being prepared
+| Batch | Input data | Spherized output file |
+| :---: | :--------: | :-------------------: |
+| 2016_04_01_a549_48hr_batch1 | DMSO normalized | 2016_04_01_a549_48hr_batch1_dmso_spherized_profiles_with_input_normalized_by_dmso.csv.gz |
+| 2016_04_01_a549_48hr_batch1 | Whole plate normalized | 2017_12_05_Batch2_dmso_spherized_profiles_with_input_normalized_by_whole_plate.csv.gz |
+| 2017_12_05_Batch2 | DMSO normalized | 2017_12_05_Batch2_dmso_spherized_profiles_with_input_normalized_by_dmso.csv.gz |
+| 2017_12_05_Batch2 | Whole plate normalized | 2017_12_05_Batch2_dmso_spherized_profiles_with_input_normalized_by_whole_plate.csv.gz |
 
-### DeepProfiler-derived Profiles
+### DeepProfiler-derived profiles
 
 TBD
 
-## Reproduce Pipeline
+## Reproduce pipeline
 
 The pipeline can be reproduced by simply executing the following:
 
@@ -53,7 +72,7 @@ python profiling_pipeline.py
 python profiling_pipeline.py --batch "2017_12_05_Batch2" --plate_prefix "BR" --well_col "Metadata_Well" --plate_col "Metadata_Plate" --extract_cell_line
 ```
 
-## Critical Details
+## Critical details
 
 There are several critical details that are important for understanding data generation and processing.
-See [`profile.py`](profile.py) for more details about the specific processing steps and decisions.
+See [`profile_cells.py`](profile_cells.py) for more details about the specific processing steps and decisions.

profiles/profiling_pipeline.py

@@ -75,7 +75,7 @@
 
     cmd = [
         "python",
-        "profile.py",
+        "profile_cells.py",
         "--sql_file",
         sql_file,
         "--batch",

spherized_profiles/scripts/nbconverted/spherize-batch-effects.py

@@ -0,0 +1,100 @@
+#!/usr/bin/env python
+# coding: utf-8
+
+# ## Adjust batch effects with a spherize transform
+# 
+# Here, we load in all normalized profiles (level 4a) data across all plates and apply a spherize transform using the DMSO profiles as the background distribution.
+# 
+# We've previously observed that sphering (aka whitening) the data successfully adjusts for technical artifacts induced by batch to batch variation and plate position effects.
+
+# In[1]:
+
+
+import os
+import pathlib
+import subprocess
+import pandas as pd
+
+from pycytominer import normalize, feature_select
+from pycytominer.cyto_utils import output, infer_cp_features
+
+
+# In[2]:
+
+
+input_dir = pathlib.Path("../profiles/")
+batches = ["2016_04_01_a549_48hr_batch1", "2017_12_05_Batch2"]
+
+suffixes = {
+    "whole_plate": "_normalized.csv.gz",
+    "dmso": "_normalized_dmso.csv.gz"
+}
+
+plates = {
+    batch: [x.name for x in pathlib.Path(f"{input_dir}/{batch}").iterdir() if ".DS_Store" not in x.name]
+    for batch in batches
+}
+
+files = {
+    batch: {
+        suffix: [pathlib.Path(f"{input_dir}/{batch}/{x}/{x}{suffixes[suffix]}") for x in plates[batch]]
+        for suffix in suffixes
+    }
+    for batch in batches
+}
+
+feature_select_ops = [
+    "variance_threshold",
+    "correlation_threshold",
+    "drop_na_columns",
+    "blacklist",
+    "drop_outliers"
+]
+
+na_cut = 0
+corr_threshold = 0.95
+outlier_cutoff = 60
+
+output_dir = "profiles"
+
+
+# In[3]:
+
+
+for batch in batches:
+    for suffix in suffixes:
+        output_file = pathlib.Path(
+            f"{output_dir}/{batch}_dmso_spherized_profiles_with_input_normalized_by_{suffix}.csv.gz"
+        )
+        print(f"Now processing {output_file}...")
+
+        profile_df = pd.concat([pd.read_csv(x) for x in files[batch][suffix]]).reset_index(drop=True)
+
+        # Perform feature selection
+        profile_df = feature_select(
+            profiles=profile_df,
+            operation=feature_select_ops,
+            na_cutoff=0,
+            corr_threshold=corr_threshold,
+            outlier_cutoff=outlier_cutoff
+        )
+
+        print(profile_df.shape)
+        profile_df.head()
+
+        spherize_df = normalize(
+            profiles=profile_df,
+            features="infer",
+            meta_features="infer",
+            samples="Metadata_broad_sample == 'DMSO'",
+            method="whiten",
+        )
+
+        print(spherize_df.shape)
+        spherize_df.head()
+
+        output(
+            df=spherize_df,
+            output_filename=output_file
+        )
+