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Created tutorial for MultiGSEA #5567

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tStehling
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Added a tutorial for MultiGSEA tool.

@tStehling tStehling requested a review from a team as a code owner November 27, 2024 12:40
@tStehling tStehling closed this Nov 27, 2024
@tStehling tStehling reopened this Nov 27, 2024
@anuprulez
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I am unsure if the tutorial should be part of the statistics category. Usually, machine learning tutorials become part of this category in GTN.

@bernt-matthias
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We are fine to move it to any other category, but none seems to fit yet. Me may create a multiomics category?

@shiltemann
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@bernt-matthias the transcriptomics topic has a "multi-omics" subsection, could we add it there for now?

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shiltemann commented Dec 4, 2024

@bernt-matthias we can then make "synthetic topic" by adding a "multi-omics" tag to all tutorials analyzing multi-omics data, and define the topic similar to the plants topic: https://github.com/galaxyproject/training-material/blob/main/metadata/plants.yaml

Then the topic will be shown on the main page, since people interested in this tutorial may not naturally go to the proteomics topic, but tutorials themselves can live in multiple topics (as they do now) What do you think?

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This sounds good to me. @tStehling could you move it?

@shiltemann do you have some links for @tStehling on how to assign tags / assign the tutorial to the sub-topic?

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Thanks a lot for your contribution @tStehling! I've left some comments below, but please let me know if anything is unclear, or if you would like some help doing it :)

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shiltemann commented Dec 4, 2024

@shiltemann do you have some links for @tStehling on how to assign tags / assign the tutorial to the sub-topic?

absolutely, @tStehling :

In the proteomics topics, the subsection id is multi-omics so you can add a tutorial to this section by adding the following to the metadata at the top of your tutorial file:

subtopic: multi-omics

And then simply add a tag of the same name. I will use this to create the "multiomics" synthetic topic later. To add a tag, add the following to the metadata of your tutorial

tags: 
  - multi-omics

(and feel free to add more tags in this list as you see fit)

tags are shown as follows under the tutorial name , and can help users identify interesting tutorials

image

@tStehling
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@shiltemann thank you for the effort.

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Very good @tStehling! Just a few mini comments, otherwise good to go from my side.


tag_based: true

gitter: galaxy-multi-omics:matrix.org
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This is different from the one above, and I think both do not exist ...
I'm also not sure if we should really create a separate room for it or if we should reuse one of the other rooms

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Thanks @tStehling! I pushed some formatting changes and left a couple small comments below

> 8. Click on `Run Tool`
>
{: .hands_on}

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would it be useful to discuss the output of the tool here? again both on technical level (what is the format, what do the contents mean?) and biological (what can we learn from the output)

@bernt-matthias
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Thanks for the comments. I will discuss with @tStehling tomorrow.


# Preparing the Data

To perform pathway enrichment with MultiGSEA, you'll need omics datasets in the file type TSV . Each individual data set contains four columns representing the feature (denoted as Symbol), the log2 fold change (logFC), the p-value (pValue), and the adjusted p-values (adj.pValue). We'll use example data provided on Zenodo.
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Maybe Sebastian can tell us a few xrefs which methods can give the needed values for Transcriptomics, Metabolomics, and Proteomics.

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bernt-matthias commented Feb 27, 2025

TODOs:

  • tools not yet on org?
  • regex search either search for [Aa]mino or enable case-insensitive
  • add two missing steps to workflow

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Lets finish this over here #5808 .. somehow I did not manage to push to this branch. Tests are passing over there.

Thanks so far @tStehling for the workd and @anuprulez and @shiltemann for the helpful comments.

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5 participants