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Deleted a stray comma and added a closing parenthesis #1

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Deleted a stray comma and added a closing parenthesis #1

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8224508
Deleted a stray comma and added a closing parenthesis
bchan Aug 1, 2019
18a8a75
Fixed the µL labels
bchan Aug 1, 2019
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14 changes: 7 additions & 7 deletions ot2_moclo_jove/miniprep/data/miniprep_template.py
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Original file line number Diff line number Diff line change
Expand Up @@ -16,22 +16,22 @@
spm = [buffers.wells(8), buffers.wells(9)]
eb = buffers.wells(10)

#Add 500 �L ETR and 20 �L Mag-Bind
p300.transfer(520, etr_mag.bottom(0.5),
#Add 500 µL ETR and 20 µL Mag-Bind
p300.transfer(520, etr_mag.bottom(0.5))
#Wait 5 min
#Magnetize and discard supernatant
#Demagnetize and add 500 �L ETR
#Demagnetize and add 500 µL ETR
#Magnetize and discard supernatant
#Demagnetize and add 700 �L VHB
#Demagnetize and add 700 µL VHB
#Magnetize and discard supernatant
#Demagnetize and add 700 �L VHB
#Demagnetize and add 700 µL VHB
#Magnetize and discard supernatant
#Demagnetize and aAdd 700 �L SPM
#Demagnetize and aAdd 700 µL SPM
#Magnetize and discard supernatant
#Wait 1 min
#Discard last bit of supernatant
#Wait 9 min
#Demagnetize and add 50-100 �L Elution Buffer (might be able to add less)
#Demagnetize and add 50-100 µL Elution Buffer (might be able to add less)
#Magnetize and remove and save supernatant (which contains dna)


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